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. 2023 Jul 12;26(8):107377. doi: 10.1016/j.isci.2023.107377

Figure 3.

Figure 3

Knockout of IRP1 reduced chemotherapy sensitivity by inhibiting ferroptosis, lipid peroxidation, and free iron accumulation

(A) The cell survival rate was measured after TMZ treatment in U87NC, U87KO, U251NC and U251KO groups. Data are represented as the mean ± SD (n = 6).

(B) Representative confocal images of the NC and KO groups stained with the PGSK (green) probe to assess intracellular iron levels. Scale bar, 100 μm.

(C) Representative confocal images of the NC and KO cells stained with the JC-1 probe (Aggregates, red; Monomers, green) to assess mitochondrial membrane potential. Scale bar, 100 μm.

(D) Representative confocal images of the NC and KO cells stained with the TMRE (red) dye to assess mitochondrial membrane potential. Scale bar, 100 μm.

(E) Representative confocal images of the NC and KO groups stained with the MitoSOX Red reagent (red) to assess mitochondrial ROS levels. Scale bar, 100 μm.

(F) Representative confocal images of the sgRNA-IRP1#1 transfected group and the sgRNA-IRP1#1 non-transfected group treated with erastin (10 μM) for 24 h stained with the DCF-DA (green) dye to assess cellular ROS. Scale bar, 100 μm.

(G) Lipid peroxidation level in the sgRNA-IRP1#1 transfected group and the sgRNA-IRP1#1 non-transfected group assessed by DCF-DA using flow cytometry immunolabelling.