Fig. 1. A subpopulation of cells exits quiescence to re-enter the cell cycle after DNA damage stress.
a Multiple cell fates arise during recovery from acute DNA damaging agents. b MCF10A cells were treated with 10 μM etoposide for 24 h before being washed and allowed to recover for 1 to 9 d. Cells were fixed and stained for Ki67, and the fraction of Ki67off cells was calculated for each condition. UT, untreated. c Dynamics of mCitrine-Ki67 with respect to cell-cycle phase in two daughter cells originating from the same mother. The top daughter proceeds through the cell cycle, while the other daughter enters a prolonged quiescence. d Experimental schematic: MCF10A cells expressing endogenously tagged mCitrine-Ki67 were treated on day -1 with 10 μM etoposide for 24 h and washed on day 0. On day 5, Ki67off cells were isolated by flow cytometry, plated, and allowed to grow for 24 h before being imaged for 96 h by time-lapse microscopy. e Single-cell traces are grouped based on their relative timing of cell-cycle re-entry from the Ki67off state; the percentage of cells in each group is indicated. 200 cell traces total are plotted in each row.