Fig. 6. p21 and Lamin B1 are short-memory markers that resolve predicted senescent cells.

a Single-cell traces from Fig. 4 were split into slow-cycling cells that completed a cell cycle during the 4 days of imaging but were CDK2^low on the final frame of the movie (N.C. at End) vs. those that were in the cell cycle on the final frame of the movie (Cyc. at End). At the end of the movie, cells were stained for p21 and Lamin B1 and the distributions of p21 and Lamin B1 were plotted for the two groups. b MCF10A cells were co-stained for Hoechst, p21, and Lamin B1 after being imaged from 6-10 d after release from a 24 h treatment of 10 μM etoposide. c The fraction of predicted-senescent cells (true positives) and corresponding false positive rates were computed for every combination of markers using a binary cutoff at the top (p21, nuclear area) or bottom (Lamin B1) quartile of signal intensity. d Topological projections of local polynomial regression modeling of the data in b for each pair of senescence biomarkers with respect to the duration CDK2^low during live-cell imaging.