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. Author manuscript; available in PMC: 2023 Oct 23.
Published in final edited form as: Cell Rep. 2023 Jan 28;42(2):112061. doi: 10.1016/j.celrep.2023.112061

Figure 4. scRNA-Seq identifies regulon activity within Lgl-KD+Upd-OE follicle cells.

Figure 4.

(A) UMAP plot to show the 42,235 ovarian-cell types grouped into 11 clusters that are identified by their unique colors and ID numbers embedded on the plot. Adjacent violin plots identify the relative expression (Y-axis) of non-epithelial cell markers that are expressed in specific clusters (X-axis). (B) Heatmap to show the activity of regulons (rows) in a random subset of 300 cells (columns) from each epithelial-cell cluster (i.e., clusters 0–2, 4, 6, 10). Both rows and columns are hierarchically clustered and the tree is shown. Individual regulons are identified by the regulating transcription factor (TF) while genes within each regulon is mentioned within parentheses next to the TFs. The regulons are colored according to their cluster IDs, which are split into the top 5 clusters. Italicized regulons include low-confidence associations. (C) UMAP plot of 42,235 single cells from Lgl-KD+Upd-OE (72h) dataset, grouped into 11 clusters. Violin plots (Right) showing cluster-specific expression of non-epithelial cell markers. (D) DNA-binding motifs related to TRE and E-box sequences are shown that correspond to the active regulons that are indicated above each logo. See also Figure S6.