KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-Fas3 (1:15) | DSHB | Cat#7G10 |
| Rat anti-shg (E-Cad, 1:20) | DSHB | Cat#DCAD2 |
| Mouse anti-Eya (1:10) | DSHB | Cat#10H6 |
| Mouse anti-Hnt (1:15) | DSHB | Cat#1G9 |
| Mouse anti-γHis2Av (1:200) | DSHB | Cat#UNC93–5.2.1 |
| Rabbit anti-pH3 (1:200) | Millipore | Cat#06–570 |
| Goat anti-CycE (1:200) | Santa Cruz | Cat#sc-15903 |
| Mouse anti-BrdU (1:20) | BD Biosciences | Cat#555627 |
| Rabbit anti-GFP (1:1000) | Abcam | Cat#ab290 |
| Phalloidin Alexa Fluor 546 (1:50) | Invitrogen | Cat#A22283 |
| Phalloidin Alexa Fluor 633 (1:50) | Invitrogen | Cat#A22284 |
| Alexa Fluor 488, 546, and 633 (1:400) | Molecular Probes | N/A |
| Rabbit anti-Cas (1:5000) | From T. Nystul | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Grace’s Insect Basal Media | VWR | Cat#45000–476 |
| Fetal Bovine Serum (FBS) | ATCC | Cat#30–2020 |
| Papain | Worthington | Cat#LK003178 |
| EBSS | TF | Cat#78154 |
| 40µL sterile cell strainer | SP Scienceware | Cat#136800040 |
| Cell counting chamber | VWR | Cat#15170–208 |
| 0.4% Trypan Blue | Sigma-Aldrich | Cat#T8154 |
| Chromium Single Cell 3’ Library & Gel Bead Kit v2 | 10x Genomics | Cat#PN-120237 |
| Chromium Single Cell A Chip Kit | 10x Genomics | Cat#PN-1000009 |
| Experimental models: Organisms/Strains | ||
| D. melanogaster: w 1118 | Deng lab stock | N/A |
| D. melanogaster: tub-GAL80 TS | BDSC | #7017 |
| D. melanogaster: UAS-lgl-RNAi: y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01905}attP40 | BDSC | #38989 |
| D. melanogaster: UAS-Dcr-2: w[1118]; P{w[+mC]=UAS-Dcr-2.D}10 | BDSC | #24651 |
| D. melanogaster: UAS-N-RNAi: y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF01637}attP2 | BDSC | #28981 |
| D. melanogaster: UAS-Stat92E-RNAi: y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00035}attP2 | BDSC | #33637 |
| D. melanogaster: UAS-Esg: y[1] w[67c23]; P{y[+mDint2] w[+mC]=EPgy2}EY10592 | BDSC | #20190 |
| D. melanogaster: UAS-Esg-RNAi: y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF03134}attP2 | BDSC | #28514 |
| D. melanogaster: UAS-lgl-RNAi | VDRC | #51247 |
| D. melanogaster: Esg:GFP | VDRC | #318400 |
| D. melanogaster: UAS-Stat92E-RNAi | VDRC | #106980 |
| D. melanogaster: tj-Gal4: y[*] w[*]; P{w[+mW.hs]=GawB}NP1624/CyO, P{w[-]=UAS-lacZ.UW14}UW14 | Kyoto | #104055 |
| D. melanogaster: UAS-NICD | Gift from Dr. T. Schüpbach | 63 |
| D. melanogaster: UAS-Upd | Gift from Dr. S. Hou | 64 |
| D. melanogaster: UAS-hop | Gift from Dr. D Harrison | 65 |
| D. melanogaster: 10Xstat92E:GFP | Gift from Dr. E Bach | 28 |
| D. melanogaster: UAS-Stat92EΔNΔC | Gift from Dr. E Bach | 66 |
| Software and algorithms | ||
| Cell Ranger v3.0.0 | 10x Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger |
| Velocyto | 67 | http://velocyto.org/ |
| Seurat v3 | 68,69 | https://satijalab.org/seurat/ |
| SCENIC | 35 | https://scenic.aertslab.org/ |
| STAR v2.7.0a | 70 | https://github.com/alexdobin/STAR |
| edgeR v3.28.1 | 71 | https://bioconductor.org/packages/edgeR/ |
| Oligonucleotides | ||
| Primer rp49 Forward: ACGCTTCAAGGGACAGTATCTG | This paper | N/A |
| Primer rp49 Reverse: AAACGCGGTTCTGCATGAG | This paper | N/A |
| Primer esg Forward: TCCATAGACCAATCGGCGTG | This paper | N/A |
| Primer esg Reverse: AATAAGCCGGCGAGATAGGC | This paper | N/A |
| Deposited Data | ||
| Raw and Analyzed Data | This paper | GSE196885 |