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. 2023 Mar 30;44(8):1625–1636. doi: 10.1038/s41401-023-01077-8

Fig. 1. TCF21 was positively correlated with vascular calcification.

Fig. 1

a, b Carotid artery atherosclerotic plaques were collected from 6 patients with carotid stenotic disease. Paraffin sections of plaques were prepared to determine SMA and TCF21 (a) or SMA and RUNX2 (b) expression by immunofluorescence staining. *P < 0.05 (n = 3). c–e HASMCs were cultured in control (Ctrl) or high phosphate medium (Pi, 3 mM) for 4 d. VSMC calcification was determined by Alizarin Red S staining (c). *P < 0.05 (n = 4). The protein and mRNA expression of TCF21, RUNX2 and BMP2 was detected by Western blotting (d) and qPCR (e), respectively. *P < 0.05 (n = 3).