Table 2.

Nb binding thermodynamics

Syringe	Sample cell	Kd (μM)	ΔG (kJ/mol)	ΔH (kJ/mol)	-TΔS (kJ/mol)	N	Increase in Kd	Decrease in ΔH (kJ/mol)
Nb714	MelBSt/Na+	0.69 ± 0.11a	−35.21 ± 0.40	−78.83 ± 4.52	43.63 ± 4.13	0.92 ± 0.09	9.2-fold	6.11
	MelBSt/Na+/Mel	6.35 ± 0.42	−29.69 ± 0.42	−72.72 ± 6.46	43.03 ± 6.28	0.96 ± 0.03
Nb725	MelBSt/Na+	1.61 ± 0.08	−33.08 ± 0.13	−88.24 ± 0.84	55.16 ± 20.72	1.01 ± 0.09	4.08-fold	4.27
	MelBSt/Na+/Mel	6.56 ± 0.83	−29.61 ± 0.31	−83.97 ± 1.42	54.35 ± 0.06	0.85 ± 0.06
Nb733	MelBSt/Na+	0.52 ± 0.07	−35.90 ± 0.32	−89.92 ± 2.64	54.01 ± 2.97	0.98 ± 0.03	11.73-fold	4.78
	MelBSt/Na+/Mel	6.10 ± 0.03	−29.77 ± 0.01	−85.14 ± 3.67	55.38 ± 3.68	0.98 ± 0.03

^aError bar, SEM; the number of tests = 2 for all.