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. 2023 Jun 23;299(8):104956. doi: 10.1016/j.jbc.2023.104956

Table 1.

IC50 and EC50 values for UNbC5-1, UNbC5-2, Ecu-mab, and RaCI3 for inhibiting complement activity and binding complement C5

Inhibitor IC50 (nM) CP EC50 (nM) C5 WT EC50 (nM) C5 R885H
UNbC5-1 137 ± 83 0.38 ± 0.20 1.5 ± 0.4
UNbC5-2 24 ± 15 0.12 ± 0.01 22.4 ± 17.0
Ecu-mab 9 ± 4 0.05 ± 0.01 ND
RaCI3 42 ± 8 - -

Binding and inhibition curves were fitted using the GraphPad Prism 9.3.0 function “[inhibitor] versus normalized response–variable slope” & “Asymmetrical Sigmoidal, 5PL, X is concentration”. IC50 and EC50 values were calculated for three individual experiments using the fitted curves, and SD were determined.