Table 2.

Binding affinities of UNbC5-1, UNbC5-2 and Ecu-mab for complement C5

Nanobody	kon (M−1s−1) × 105	koff (s−1) × 10−6	KD (pM)
UNbC5-1	2.713 ± 0.002	3.254 ± 0.06	119.9 ± 0.2
UNbC5-2	2.937 ± 0.008	0.227 ± 0.03	7.7 ± 0.1
Ecu-mab	3.661 ± 0.003	3.810 ± 0.10	104.0 ± 0.4

Nanobody and Ecu-mab affinities were determined by SPR using C5 as analyte and nanobodies and Ecu-mab as a ligand. All measurements were performed in duplicate. Kinetics were determined using Scrubber 2.0 (BioLogic Software).