Table 3.
Cryo-EM data collection, refinement, and validation statistics
| Data collection and processing | C5:UNbC5-1:UNbC5-2 PDB-8CML, EMDB-16730 |
|---|---|
| Microscope | Talos Arctica |
| Camera | Gatan K2 Summit + GIF |
| Magnification | 130,000 |
| Voltage (kV) | 200 |
| Exposure time frame/total (s) | 0.2/8.0 |
| Number of frames | 40 |
| Electron exposure (e−/Å2) | 54–57 |
| Defocus range (μm) | −0.8 to −2.6 |
| Pixel size (Å) | 1.04 |
| Micrographs (no.) | 1253; 1840 |
| Initial particle images (no.) | 1,483,794 |
| Final particles images (no.) | 193,009 |
| Map Resolution (Å) 0.143 FSC threshold |
3.60 |
| Map resolution range (Å) | 3.1–7.4 |
| Refinement | |
| Model Resolution (Å) 0.143 FSC threshold | 3.60 |
| Map sharpening B factor (Å2) | −120.3 |
| Model composition | |
| Non-hydrogen atoms | 13,594 |
| Protein residues | 1728 |
| Ligands | NAG: 2 |
| B factors (Å2) | |
| Protein | 52.71 |
| Ligands | 50.80 |
| RMS deviations | |
| Bond lengths (Å) | 0.005 |
| Bond angles (o) | 0.842 |
| Validation | |
| MolProbity score | 2.41 |
| Clashscore | 13.01 |
| Rotamer outliers (%) | 2.91 |
| Ramachandran plot | |
| Favored (%) | 93.35 |
| Allowed (%) | 6.01 |
| Outliers (%) | 0.64 |