Table 4.

Constructs and sequences used for protein purifications

#	Description/sequence
1	pcDNA34-Cystatin(S)-C5-Linker-LPETG-HIS vector sequence from the XbaI up to the AgeI sequence, in which the NheI and NotI site are depicted in gray, the start (ATG) and stop (TAA) codon are underlined and the 13 bp and 15 bp sequences adjacent to the XbaI and AgeI sites are in bold.
	TCTAGAGGATCGAACCCTTACCACCATGGCAAGACCTCTTTGTACCCTGCTTCTGCTCATGGCGACACTGGCTGGGGCCCTCGCCGCTAGCCAGGAGCAAACATATGTCATTTCAGCACCAAAAATATTCCGTGTTGGAGCATCTGAAAATATTGTGATTCAAGTTTATGGATACACTGAAGCATTTGATGCAACAATCTCTATTAAAAGTTATCCTGATAAAAAATTTAGTTACTCCTCAGGCCATGTTCATTTATCCTCAGAGAATAAATTCCAAAACTCTGCAATCTTAACAATACAACCAAAACAATTGCCTGGAGGACAAAACCCAGTTTCTTATGTGTATTTGGAAGTTGTATCAAAGCATTTTTCAAAATCAAAAAGAATGCCAATAACCTATGACAATGGATTTCTCTTCATTCATACAGACAAACCTGTTTATACTCCAGACCAGTCAGTAAAAGTTAGAGTTTATTCGTTGAATGACGACTTGAAGCCAGCCAAAAGAGAAACTGTCTTAACTTTCATAGATCCTGAAGGATCAGAAGTTGACATGGTAGAAGAAATTGATCATATTGGAATTATCTCTTTTCCTGACTTCAAGATTCCGTCTAATCCTAGATATGGTATGTGGACGATCAAGGCTAAATATAAAGAGGACTTTTCAACAACTGGAACCGCATATTTTGAAGTTAAAGAATATGTCTTGCCACATTTTTCTGTCTCAATCGAGCCCGAATATAATTTCATCGGCTACAAGAACTTTAAGAACTTCGAGATTACCATCAAGGCCAGATACTTCTATAATAAGGTAGTCACTGAGGCTGACGTTTATATCACATTTGGAATAAGAGAAGACTTAAAAGATGATCAAAAAGAAATGATGCAAACAGCAATGCAAAACACAATGTTGATAAATGGAATTGCTCAAGTCACATTTGATTCTGAAACAGCAGTCAAAGAACTGTCATACTACAGTTTAGAAGATTTAAACAACAAGTACCTTTATATTGCTGTAACAGTCATAGAGTCTACAGGTGGATTTTCTGAAGAGGCAGAAATACCTGGCATCAAATATGTCCTCTCTCCCTACAAACTGAATTTGGTTGCTACTCCTCTTTTCCTGAAGCCTGGGATTCCATATCCCATCAAGGTGCAGGTTAAAGATTCGCTTGACCAGTTGGTAGGAGGAGTCCCAGTAACACTGAATGCACAAACAATTGATGTAAACCAAGAGACATCTGACTTGGACCCAAGCAAAAGTGTAACACGTGTTGATGATGGAGTAGCTTCCTTTGTGCTTAATCTCCCATCTGGAGTGACGGTGCTGGAGTTTAATGTCAAAACTGATGCTCCAGATCTTCCAGAAGAAAATCAGGCCAGGGAAGGTTACCGAGCAATAGCATACTCATCTCTCAGCCAAAGTTACCTTTATATTGATTGGACTGATAACCATAAGGCTTTGCTAGTGGGAGAACATCTGAATATTATTGTTACCCCCAAAAGCCCATATATTGACAAAATAACTCACTATAATTACTTGATTTTATCCAAGGGCAAAATTATCCACTTTGGCACGAGGGAGAAATTTTCAGATGCATCTTATCAAAGTATAAACATTCCAGTAACACAGAACATGGTTCCTTCATCCCGACTTCTGGTCTATTACATCGTCACAGGAGAACAGACAGCAGAATTAGTGTCTGATTCAGTCTGGTTAAATATTGAAGAAAAATGTGGCAACCAGCTCCAGGTTCATCTGTCTCCTGATGCAGATGCATATTCTCCAGGCCAAACTGTGTCTCTTAATATGGCAACTGGAATGGATTCCTGGGTGGCATTAGCAGCAGTGGACAGTGCTGTGTATGGAGTCCAAAGAGGAGCCAAAAAGCCCTTGGAAAGAGTATTTCAATTCTTAGAGAAGAGTGATCTGGGCTGTGGGGCAGGTGGTGGCCTCAACAATGCCAATGTGTTCCACCTAGCTGGACTTACCTTCCTCACTAATGCAAATGCAGATGACTCCCAAGAAAATGATGAACCTTGTAAAGAAATTCTCAGGCCAAGAAGAACGCTGCAAAAGAAGATAGAAGAAATAGCTGCTAAATATAAACATTCAGTAGTGAAGAAATGTTGTTACGATGGAGCCTGCGTTAATAATGATGAAACCTGTGAGCAGCGAGCTGCACGGATTAGTTTAGGGCCAAGATGCATCAAAGCTTTCACTGAATGTTGTGTCGTCGCAAGCCAGCTCCGTGCTAATATCTCTCATAAAGACATGCAATTGGGAAGGCTACACATGAAGACCCTGTTACCAGTAAGCAAGCCAGAAATTCGGAGTTATTTTCCAGAAAGCTGGTTGTGGGAAGTTCATCTTGTTCCCAGAAGAAAACAGTTGCAGTTTGCCCTACCTGATTCTCTAACCACCTGGGAAATTCAAGGCGTTGGCATTTCAAACACTGGTATATGTGTTGCTGATACTGTCAAGGCAAAGGTGTTCAAAGATGTCTTCCTGGAAATGAATATACCATATTCTGTTGTACGAGGAGAACAGATCCAATTGAAAGGAACTGTTTACAACTATAGGACTTCTGGGATGCAGTTCTGTGTTAAAATGTCTGCTGTGGAGGGAATCTGCACTTCGGAAAGCCCAGTCATTGATCATCAGGGCACAAAGTCCTCCAAATGTGTGCGCCAGAAAGTAGAGGGCTCCTCCAGTCACTTGGTGACATTCACTGTGCTTCCTCTGGAAATTGGCCTTCACAACATCAATTTTTCACTGGAGACTTGGTTTGGAAAAGAAATCTTAGTAAAAACATTACGAGTGGTGCCAGAAGGTGTCAAAAGGGAAAGCTATTCTGGTGTTACTTTGGACCCTAGGGGTATTTATGGTACCATTAGCAGACGAAAGGAGTTCCCATACAGGATACCCTTAGATTTGGTCCCCAAAACAGAAATCAAAAGGATTTTGAGTGTAAAAGGACTGCTTGTAGGTGAGATCTTGTCTGCAGTTCTAAGTCAGGAAGGCATCAATATCCTAACCCACCTCCCCAAAGGGAGTGCAGAGGCGGAGCTGATGAGCGTTGTCCCAGTATTCTATGTTTTTCACTACCTGGAAACAGGAAATCATTGGAACATTTTTCATTCTGACCCATTAATTGAAAAGCAGAAACTGAAGAAAAAATTAAAAGAAGGGATGTTGAGCATTATGTCCTACAGAAATGCTGACTACTCTTACAGTGTGTGGAAGGGTGGAAGTGCTAGCACTTGGTTAACAGCTTTTGCTTTAAGAGTACTTGGACAAGTAAATAAATACGTAGAGCAGAACCAAAACAGCATCTGCAACAGCTTATTGTGGCTAGTTGAGAATTATCAATTAGATAATGGATCTTTCAAGGAAAATTCACAGTATCAACCAATAAAATTACAGGGTACCTTGCCTGTTGAAGCCCGAGAGAACAGCTTATATCTTACAGCCTTTACTGTGATTGGAATTAGAAAGGCTTTCGATATATGCCCCCTGGTGAAAATCGACACAGCTCTAATTAAAGCTGACAACTTTCTGCTTGAAAATACACTGCCAGCCCAGAGCACCTTTACATTGGCCATTTCTGCGTATGCTCTTTCCCTGGGAGATAAAACTCACCCACAGTTTCGTTCAATTGTTTCAGCTTTGAAGAGAGAAGCTTTGGTTAAAGGTAATCCACCCATTTATCGTTTTTGGAAAGACAATCTTCAGCATAAAGACAGCTCTGTACCTAACACTGGTACGGCACGTATGGTAGAAACAACTGCCTATGCTTTACTCACCAGTCTGAACTTGAAAGACATCAACTACGTGAACCCAGTCATCAAATGGCTATCAGAAGAGCAGAGGTATGGAGGTGGCTTTTATTCAACCCAGGACACAATCAATGCCATTGAGGGCCTGACGGAATATTCACTCCTGGTTAAACAACTCCGCTTGAGTATGGACATCGATGTTTCTTACAAGCATAAAGGTGCCTTACATAATTATAAAATGACAGACAAGAATTTCCTTGGGAGGCCAGTAGAGGTGCTTCTCAATGATGACCTCATTGTCAGTACAGGATTTGGCAGTGGCTTGGCTACAGTACATGTAACAACTGTAGTTCACAAAACCAGTACCTCTGAGGAAGTTTGCAGCTTTTATTTGAAAATCGATACTCAGGATATTGAAGCATCCCACTACAGAGGCTACGGAAACTCTGATTACAAACGCATAGTAGCATGTGCCAGCTACAAGCCCAGCAGGGAAGAATCATCATCTGGCTCCTCTCATGCGGTGATGGACATCTCCTTGCCTACTGGAATCAGTGCAAATGAAGAAGACTTAAAAGCCCTTGTGGAAGGGGTGGATCAACTATTCACTGATTACCAAATCAAAGATGGACATGTTATTCTGCAACTGAATTCGATTCCCTCCAGTGATTTCCTTTGTGTACGATTCCGGATATTTGAACTCTTTGAAGTTGGGTTTCTCAGTCCTGCCACTTTCACAGTGTACGAATACCACAGACCAGATAAACAGTGTACCATGTTTTATAGCACTTCCAATATCAAAATTCAGAAAGTCTGTGAAGGAGCCGCGTGCAAGTGTGTAGAAGCTGATTGTGGGCAAATGCAGGAAGAATTGGATCTGACAATCTCTGCAGAGACAAGAAAACAAACAGCATGTAAACCAGAGATTGCATATGCTTATAAAGTTAGCATCACATCCATCACTGTAGAAAATGTTTTTGTCAAGTACAAGGCAACCCTTCTGGATATCTACAAAACTGGGGAAGCTGTTGCTGAGAAAGACTCTGAGATTACCTTCATTAAAAAGGTAACCTGTACTAACGCTGAGCTGGTAAAAGGAAGACAGTACTTAATTATGGGTAAAGAAGCCCTCCAGATAAAATACAATTTCAGTTTCAGGTACATCTACCCTTTAGATTCCTTGACCTGGATTGAATACTGGCCTAGAGACACAACATGTTCATCGTGTCAAGCATTTTTAGCTAATTTAGATGAATTTGCCGAAGATATCTTTTTAAATGGATGCGCGGCCGCAGGAGGAGGCGGTTCCCTGCCTGAAACGGGCGGTCACCATCACCATCACCATTAAAAGGGTTCGATCCCTACCGGT
2	pcDNA34-Cystatin(S)-RacI3-LPETG-HIS vector sequence from the XbaI up to the AgeI sequence, in which the NheI and NotI site are depicted in gray, the start (ATG) and stop (TAA) codon are underlined.
	TCTAGAGGATCGAACCCTTACCACCATGGCAAGACCTCTTTGTACCCTGCTTCTGCTCATGGCGACACTGGCTGGGGCCCTCGCCGCTAGCAGCGGAGAGTCTCAGTCAATTCAGAGGAAAGGGCAATGTGAGGAAGTCATTTGTCACCGAAAATTGAACCATCTCGGGGAAAGGGTCACCAGCGGATGCCCGACCGGTTGCCTCTGTGTCATAAGGGAGCCAGATAATGTCGATAATGCTAACGGGACATGCTATGCTCTGATGAGCAGTACGACCACGACCACAACAACACCGGACGGCACAACCACCAGTGAGGAGGAGGAAGCGGCCGCACTGCCTGAAACGGGCGGTCACCATCACCATCACCATTAAAAGGGTTCGATCCCTACCGGT
3	Ecu-mab VH+ constant region gBlock, with the HAVT20 signal peptide depicted in bold:
	GATCCAGCCTCCGGACTCTAGAGGATCGAACCCTTGAATTCACCACCATGGCTTGCCCTGGCTTCCTGTGGGCATTGGTAATCAGCACTTGCCTGGAGTTTTCTATGGCTCAGGTACAACTTGTGCAATCTGGGGCTGAAGTTAAAAAACCAGGTGCTAGTGTTAAAGTGAGCTGCAAAGCCAGCGGTTACATCTTCAGTAACTATTGGATACAATGGGTGCGACAAGCGCCCGGACAAGGCTTGGAGTGGATGGGCGAAATTCTGCCCGGAAGCGGTAGCACAGAATACACTGAGAACTTTAAAGATCGAGTAACGATGACCCGAGATACCAGTACTTCTACGGTTTATATGGAACTGTCATCACTGCGCTCTGAAGACACCGCGGTGTATTACTGTGCCAGGTATTTTTTTGGTAGTTCCCCTAACTGGTACTTCGACGTGTGGGGGCAGGGCACGCTGGTTACGGTCAGCTCCGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCCGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCTGGATTGTATTCTTTGTCATCCGTCGTAACGGTGCCCAGTAGCAATTTCGGCACACAAACATATACTTGCAATGTAGATCATAAGCCAAGCAACACCAAGGTCGATAAGACCGTGGAAAGAAAGTGTTGCGTCGAGTGTCCTCCTTGCCCGGCACCACCCGTGGCGGGTCCCAGTGTCTTCCTGTTCCCACCGAAACCCAAGGATACTCTCATGATATCTCGAACCCCCGAGGTGACATGCGTCGTCGTGGATGTCTCTCAGGAAGACCCGGAGGTCCAATTTAACTGGTATGTTGATGGAGTAGAAGTACATAATGCAAAGACCAAACCACGGGAGGAGCAGTTTAATAGCACCTACAGAGTAGTGAGCGTACTGACGGTGCTTCATCAAGACTGGCTCAACGGAAAAGAATACAAATGTAAAGTGAGTAATAAGGGCCTTCCTAGCTCTATCGAAAAGACGATTTCAAAGGCCAAAGGCCAGCCCAGAGAGCCTCAAGTTTACACGCTTCCGCCATCCCAAGAAGAAATGACAAAGAACCAAGTATCACTCACGTGTTTGGTTAAAGGGTTCTATCCGTCAGACATCGCGGTAGAGTGGGAGTCTAATGGACAGCCTGAGAACAACTATAAAACCACTCCACCCGTTCTCGATTCCGATGGCAGCTTTTTCTTGTACAGTCGCCTGACCGTTGACAAAAGCCGCTGGCAGGAGGGCAACGTGTTCTCCTGTAGTGTGATGCACGAGGCGCTTCACAATCACTACACACAAAAGTCACTTTCTTTGAGCCTGGGCAAATGAACCGGTTAGTAATGAGTTTGATATCTC
4	Ecu-mab VL+constant region gBlock, with the HAVT20 signal peptide depicted in bold:
	GACCGATCCAGCCTCCGGACTCTAGAGGATCGAACCCTTGAATTCACCACCATGGCTTGCCCTGGCTTCCTGTGGGCATTGGTAATCAGCACTTGCCTGGAGTTTTCTATGGCTGATATACAGATGACACAGTCTCCCAGTAGTTTGTCTGCTTCAGTAGGGGATCGAGTCACGATTACATGCGGCGCTTCCGAAAATATCTATGGAGCACTCAACTGGTATCAGCAGAAACCCGGAAAGGCGCCAAAGTTGCTGATTTACGGGGCGACCAACCTGGCTGACGGGGTGCCTAGCAGGTTTAGCGGATCTGGCAGCGGCACAGACTTCACTCTTACAATTTCTTCTCTCCAACCGGAGGACTTCGCTACTTACTACTGCCAAAATGTGCTGAATACGCCATTGACGTTCGGCCAGGGAACCAAGGTCGAGATTAAGCGTACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGAAGGGTTCGATCCCTACCGGTTAGTAATGAGTTTGATATCTC
5	Ecu-Fab VH+constant Fab region gBlock, with the HAVT20 signal peptide depicted in bold:
	GATCCAGCCTCCGGACTCTAGAGGATCGAACCCTTGAATTCACCACCATGGCTTGCCCTGGCTTCCTGTGGGCATTGGTAATCAGCACTTGCCTGGAGTTTTCTATGGCTCAGGTACAACTTGTGCAATCTGGGGCTGAAGTTAAAAAACCAGGTGCTAGTGTTAAAGTGAGCTGCAAAGCCAGCGGTTACATCTTCAGTAACTATTGGATACAATGGGTGCGACAAGCGCCCGGACAAGGCTTGGAGTGGATGGGCGAAATTCTGCCCGGAAGCGGTAGCACAGAATACACTGAGAACTTTAAAGATCGAGTAACGATGACCCGAGATACCAGTACTTCTACGGTTTATATGGAACTGTCATCACTGCGCTCTGAAGACACCGCGGTGTATTACTGTGCCAGGTATTTTTTTGGTAGTTCCCCTAACTGGTACTTCGACGTGTGGGGGCAGGGCACGCTGGTTACGGTCAGCTCCGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGGAGGTGGAGGCAGTCTGCCGGAGACCGGAGGGCACCATCACCATCACCATTGAAAGGGTTCGATCCCTACCGGTTAGTAATGAGTTTGATATC