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Effect of increased osmolality on the growth rates of L. lactis NCDO763. Cells were grown in CDM without (○) or with (●) 1 mM betaine. The osmolality was increased by the addition of NaCl. The inset shows a growth curve (▵) in CDM containing 0.3 M NaCl and 1 mM betaine, and the intracellular betaine content (▴) was measured by HPLC analysis at different times.
