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. 2023 Jul 13;145(29):15936–15950. doi: 10.1021/jacs.3c03749

Figure 7.

Figure 7

(a) Competition binding assay using thioflavin T (ThT). There is an increase in the fluorescence intensity when ThT binds to an amyloid fibril. Binding of a competing ligand L (E570) is detected by the decrease in fluorescence intensity when ThT is displaced. (b) Fluorescence titration of ThT into a solution of Aβ(1–42) fibrils (500 nM) in 1× PBS buffer (pH 7.4, 25 °C), followed by a titration of the competing ligand. Spectra were recorded using λex = 440 nm, and the fluorescence emission was monitored at λem = 483 nm. The experimental measurements are shown as points (error bars represent the 95% confidence interval calculated from at least three independent experiments), and the lines are the best fits to eq 2 with log(Kd(ThT)/M) = −6.7 and log(Kd(L)/M) = −7.6.