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Analysis of MNV RNA transcript integrity by nondenaturing gel electrophoresis. In vitro transcribed RNA (IVT), purified RNA (Pure), and capped RNA (Cap) products are run at 120 V for 10 min on a nondenaturing 1% agarose gel (TAE buffer) in parallel to a GeneRuler™ 1 kb Plus DNA Ladder (1 kb, lane 1, not representative of RNA sizes).
