Table 2:
Reaction Setup for NheI Linearization of MNV cDNA
| Components | Final Concentration | Volume (μl) |
|---|---|---|
| 10x CutSmart Buffer (NEB) | 1x | 20 |
| NheI-HF 20 U/μl (NEB) | 0.3 U/μl | 3 |
| cDNA (pT7:MNV3’Rz) | 100 ng/μl | Variable |
| ddH2O | -- | add to 200 |
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