Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Reaction set up for in vitro transcription of linearized MNV cDNA
| Reagent | Final Concentration | Volume (μl) |
|---|---|---|
| 10x Buffer | 1x | 4.0 |
| rNTPs (10 mM each) | 0.5 mM each | (2.0 each) 8.0 |
| T7 Enzyme (15 U/μl) | 0.75 U/μl | 2.0 |
| Linear DNA | 100 ng/μl | (3–4 μg) ~10–15 |
| ddH2ORNase-free | -- | Up to 40 |
