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. 2023 Jul 28;19:73. doi: 10.1186/s13007-023-01049-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 10 — Localisation of co-expressed MADS-box proteins in N. benthamiana leaf cells. A Localisation of the AP3/PI heteromer visualized by BiFC. BiFC did not interfere with AP3/PI translocation to the nucleus or nucleolus. B–D Localisation of SEP3-mCh, NLS-mCh and AP1-mCh respectively. E–E’’: Co-expression of AP3/PI and SEP3 (E Signal from AP3/PI, E’: Signal from SEP3, E’’ Merged signal). SEP3 accumulated at the nucleolus and fully co-localized with AP3/PI. F–F’’ Co-expression of AP3/PI and AP1 (F Signal from AP3/PI, F’ Signal from AP1, F’’ Merged signal). AP1 weakly accumulated at in the presence of AP3/PI. G–G’’ Co-expression of AP3/PI and NLS-mCh (G Signal from AP3/PI, G’ Signal from NLS-mCh, G’’ Merged signal). NLS-mCh localisation did not change in the presence of AP3/PI. H–H’’’ Co-expression of AP3/PI with SEP3 and AP1 (E Signal from AP3/PI, E’ Signal from SEP3, E’’ Signal from AP1 E’’’ Merged signal). Expression of SEP3 with AP3/P and AP1 lead to strong re-localisation of AP1 to the nucleolus and full co-localisation of all four MADS-domain proteins. (Scale bars: 10 µm)