Skip to main content
. 2023 Jul 14;14:1215900. doi: 10.3389/fphys.2023.1215900

FIGURE 6.

FIGURE 6

Effect of NS-398 on baseline colonic smooth muscle contractile activity in Crohn’s-like colitis rats. NS-398 (5 mg/kg, i. p. daily) and vehicle control (DMSO saline) were administered daily to sham and TNBS-induced Crohn’s-like colitis rats. Colonic smooth muscle strips were prepared and mounted at 1 g of tension for 40–50 min in tissue bath. The integral contractile activity (area under contractions, AUC) was determined with the average of two measurements of activity for 2 min. With vehicle treatment, TNBS colitis rats demonstrated a significantly lower baseline colonic contractile activity than in sham rats at both P and I sites (A-a and A-b, respectively). NS-398 treatment significantly improved colonic baseline activity in TNBS colitis rats at both P-site and I-site (A–a, A–b). However, even with NS-398 treatment, the baseline activity was still reduced in TNBS rats compared to sham control at the I-site (A,B). Representative traces for all groups are shown in (B). The number at the right bottom of each trace is the tissue weight of the muscle strip. The vertical and horizontal bars of the insert at the right bottom of panel B indicate 1 g of tension and 1 min of time, respectively for all traces. N = 5 rats. p values are given for the comparisons in (A). S, sham; T, TNBS treated rats (Crohn’s-like colitis); Veh, vehicle.