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. 2023 Jul 27;28:60. doi: 10.1186/s11658-023-00467-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The activity of NFATc1 induced by DYRK1A in early puberty. A Western blot show the expression of NFATc1 related phosphokinases and their phosphorylation in VS of PND-25 and PND-35. B Western blot show the phosphorylation of NFATc1 affected by DYRK1A inhibitor. C IF show the localization of NFATc1 and phosphorylated NFATc1 in VS of PND-25, PND25 with DYRK1A inhibitor and PND-35 (magnification ×200). The SG specimens from two individuals were used for western blot. The SG specimens from three individuals were used for immunofluorescence