Fig. 4.

Differential analysis of the intestinal bacterial cohorts between the two phases of the pre-immune experiment. Random forests were sampled to evaluate significant discriminant features from both phases at the age of 8 days (A) and 15 days (B) using the trans_diff function in R package microeco. (C) A LDA was performed for 15 days old using LEfSe. The length of the bar represents the log10 transformed LDA score, indicated by the vertical dashed line. The threshold of the log LDA score for discriminating features is set at 2.0. 08, 15, Life stage (in number of days) at which analysis was performed; BLES, Fengqiang Shengtai quadruple probiotic preparation; CAG-56, Firmicutes bacterium CAG-56; Con, control group; Gini, Gini index; LDA, linear discriminant analysis; LEfSe, linear discriminant analysis effect size analysis; Pre, pre-treatment groups; Vac, vaccine against avian coccidiosis