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Extraction of chenodeoxycholic acid (CDCA) using different methods.
| Factors | Existing method | Simplified method | Enzymatic method |
|---|---|---|---|
| Extraction time (h) | 24 | 8 | 2 |
| Hydrolysis reaction time (h) | 8 | 6 | 1 |
| Temperature (°C) | 121 | 60 | 37 |
| Reusability | X | X | Ｏ (Possibility) |
| Wastewater | Alkaline | Alkaline | X |
| Yield (%) | 92.09 ± 3.13a | 82.24 ± 5.91b | 58.18 ± 14.03c |
Data are presented as mean ± SD (n = 3).
a, b, c Means within same parameter are significantly different (P < 0.05).
