. 2023 Jul 11;12(7):985. doi: 10.3390/biology12070985

Table A1.

Characteristics of sets of primers used for qPCR analysis.

Set ^a	Primer Pair ^b	Sequence (5′ to 3′) ^c	Target Genes	qPCR Product Size	Melting Point ^d (°C)
1	DSR1F	ACSCACTGGAAGCACG	dsrA	222	87
1	RH3-dsr-R	gGTGGAGCCGTGCATGTT	dsrA	222	87
2	RH1-dsr-F	GCCGTTACTGTGACCAGCC	dsrA	164	87
2	RH3-dsr-R	gGTGGAGCCGTGCATGTT	dsrA	164	87
3	APS7-F	GGGYCTKTCCGCYATCAAYACATGA	apsA	279	89
3	RH2-aps-R	ATCATGATCTGCCAgCGgCCGGA	apsA	279	89
4	RH1-aps-F	CGCGAAGACCTKATCTTCGAC	apsA	191	90
4	RH2-aps-R	ATCATGATCTGCCAgCGgCCGGA	apsA	191	90

^a Sets of primers were designed and evaluated in the study of Ben-Dov et al. [36]. ^b F: direct or forward primer. R: reverse primer. ^c Boldface represents mixed bases, also known as degenerate or wobble bases. Lowercase represents bases that do not match appropriate sequences. ^d Values of this study.