Table 5.
Primer sequences and PCR cycling conditions.
Target Strain | Target Gene | Primer Sequence (51–31) | PCR Cycling Condition | Product Size (bp) | Reference |
---|---|---|---|---|---|
Klebsiella genus | gyrA | F:CGCGTACTATACGCCATGAACGTA R:ACCGTTGATCACTTCGGTCAGG |
‘‘94 °C (5 min), 94 °C (30 s), 55 °C (45 s), 72 °C (45 s), 72 °C (10 min) × 35 cycles” | 441 | [16] |
K. pneumoniae | magA | F:ATTTGAAGAGGTTGCAAACGAT R: TTCACTCTGAAGTTTTCTTGTGTTC |
‘‘94 °C (5 min), 94 °C (30 s), 55 °C (30 s), 72 °C (45 s), 72 °C (10 min) × 30 cycles” | 130 | [17] |