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The primer sequence information of MyoD and Pax7 for RT-PCR analysis.
| Gene Symbol | Primer Sequence (5′→3′) | Product Length (bp) |
Annealing Temperature (°C) | Accession No. |
|---|---|---|---|---|
| MyoD | F: ACTACAGCGGGGAGTCAGAT | 149 | 57 | NM_204214.3 |
| R: CCCATGCTTTGGGTCATTTGG | ||||
| Pax7 | F: TCGATTAGCCGTGTGCTACG | 108 | 59.2 | NM_205065.1 |
| R: GCCATCTATGCTGTGCTTGG | ||||
| GAPDH | F: GGGGAAAGTCATCCCTGAGC | 145 | 60 | NM_204305.1 |
| R: AGCAGCCTTCACTACCCTCT |
F: Forward primer; R: Reverse primer.
