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Two-step nitration of tyrosine (Tyr) in the presence of decomposition products of peroxynitrite (ONOO−): OH•, CO3•−, and •NO2. In the first step, the interaction of OH•/CO3•− with tyrosine takes place, resulting in the formation of a tyrosyl radical (Tyr•). In the second reaction, Tyr• and •NO2 form 3-nitrotyrosine.
