Figure 8.

Different roles of time of culturing and substrate in MMPs gene expression in LoVo-S and LoVo-R cultivated on Matrigel or type I Collagen meshwork after 3 and 24 h. Over time, LoVo-S cultured on type I collagen showed a decrease in E-cadherin, SNAIL, MMP-2, -9, and -14 expression and an increase in vimentin. The decrease in MMP-2 expression seems to be related to duration but is particularly influenced by type I Collagen substrate. The LoVo-R cells cultured on the same substrate exhibited a decrease in SNAIL, MMP-9, -14, and HPSE expression but an increase in E-cadherin and MMP-2 expression, which were also related to the type of substrate. The size of the arrows is approximately related to the range of increase/decrease.