Table 2.
Cell membrane fluidity and surface charge of study strains.
| S. oralis Strains | CM Fluidity: PI Value (Mean ± SD) |
Surface Charge: % of Cyt C in Supernatant (Mean ± SD) |
BODIPY-DAP Binding: Fluorescence Intensity (Mean ± SD) |
|---|---|---|---|
| 73 | 0.298 ± 0.01 | 76 ± 3 | 553 ± 28 |
| 73-D2 | 0.271 ± 0.01 * | 61 ± 1 * | 592 ± 17 * |
| 205 | 0.268 ± 0.01 | 62 ± 8 | 405 ± 3 |
| 205-D2 | 0.269 ± 0.01 | 58 ± 7 | 430 ± 24 |
Data represent the mean (±SD) of three independent experiments on different days. Statistical significance (*) for D2 strains relative to their HLDR and non-HLDR parental strains were assessed using Student’s t-test (p ≤ 0.05). * p < 0.05 D2 vs. respective parental strains. An increased amount of cytC in the supernatant correlates to a greater positively charged bacterial surface; a lower PI value equates to a higher degree of CM fluidity.