Table 5.
Summary of amino acid changes (n = 64) in 205-D2 vs. parental 205 S. oralis.
| Predicted Amino Acid Change a | Predicted Protein Function | Gene ID |
|---|---|---|
| Glu144Gln | tagatose-bisphosphate aldolase | gene-EL140_RS04205 |
| Glu458Asp | fibronectin-binding SSURE repeat-containing protein | gene-EL140_RS00420 |
| Lys3Glu | PspC domain-containing protein | gene-EL140_RS00510 |
| Met6Leu | ribonuclease M5 | gene-EL140_RS01180 |
| Asn331Gln, Val2276Leu, His/Lys331Gln | accessory Sec-dependent serine-rich glycoprotein adhesin | gene-EL140_RS01855 |
| leu51Met, Ser151Ala, Arg165Lys | serine O-acetyltransferase | gene-EL140_RS02135 |
| Asn8Asp | nucleoside phosphorylase | gene-EL140_RS00405 |
| Asp43Glu | DUF1307 domain-containing protein | gene-EL140_RS02430 |
| Arg6Trp | DUF6287 domain-containing protein | gene-EL140_RS02620 |
| 599insAT | N-acetylmuramoyl-L-alanine amidase | gene-EL140_RS02630 |
| Ile53Val, His139Arg, Ser690Phe, Leu694Val | heavy metal translocating P-type ATPase | gene-EL140_RS00600 |
| Asn295Ser | hypothetical protein | gene-EL140_RS00190 |
| Gln992His | carbamoyl-phosphate synthase large subunit | gene-EL140_RS03330 |
| Glu438ins, GluGlnPro439HisProThr | DNA primase | gene-EL140_RS03860 |
| Arg51His, AspGln566GlyLeu, Val570Ala | glycoside hydrolase family 13 protein | gene-EL140_RS04725 |
| Glu229Lys | YjjG family noncanonical pyrimidine nucleotidase | gene-EL140_RS04975 |
| Asn142Asp | SUF system NifU family Fe-S cluster assembly protein | gene-EL140_RS05575 |
| Ala17Val | aminoglycoside 6-adenylyltransferase | gene-EL140_RS05640 |
| Phe93Cys | VanZ family protein | gene-EL140_RS05855 |
| Glu47Lys | UPF0223 family protein | gene-EL140_RS06150 |
| Asp65Glu | SDR family oxidoreductase | gene-EL140_RS06415 |
| Val294Ile | ribonuclease Z | gene-EL140_RS06420 |
| LeuAla83LeuThr, GlyLeu86AlaIle | hypothetical protein | gene-EL140_RS00190 |
| Asn350Lys | G5 domain-containing protein | gene-EL140_RS06930 |
| Ser824Asn, Thr1012Asn, Thr843Ile, Thr820Asn | G5 domain-containing protein | gene-EL140_RS06930 |
| Val1055Ala | exo-alpha-sialidase | gene-EL140_RS07065 |
| Val138Met | DUF421 domain-containing protein | gene-EL140_RS07435 |
| Asp1472Glu | glycoside hydrolase N-terminal domain-containing protein | gene-EL140_RS08115 |
| Asp871Glu | DNA-directed RNA polymerase subunit beta’ | gene-EL140_RS08440 |
| Cys610Gly | magnesium-translocating P-type ATPase | gene-EL140_RS08825 |
| Met267Ile | D-alanyl-lipoteichoic acid biosynthesis protein DltD | gene-EL140_RS09230 |
| Arg74Ser | hypothetical protein | gene-EL140_RS00190 |
| Ala862Thr | LPXTG cell wall anchor domain-containing protein | gene-EL140_RS06375 |
| Val137Leu | NAD(P)H-dependent glycerol-3-phosphate dehydrogenase | gene-EL140_RS00645 |
| Thr718Ala | HAD-IC family P-type ATPase | gene-EL140_RS01060 |
| LysValLeuValGly150AsnAlaLeuThrIle, Ala159Ser | sugar transferase | gene-EL140_RS01435 |
| IleAspLeu211ValAlaIle | metal ABC transporter ATP-binding protein | gene-EL140_RS02210 |
| Asn117Ser | DNA/RNA non-specific endonuclease | gene-EL140_RS01295 |
| Leu6Gln | NAD-dependent protein deacylase | gene-EL140_RS03745 |
| Asp74His | nucleotidyltransferase | gene-EL140_RS01975 |
| Gly14Ala | MBL fold metallo-hydrolase | gene-EL140_RS02220 |
| Lys12Gln | MmcQ/YjbR family DNA-binding protein | gene-EL140_RS05435 |
| Ala177Val | DNA topology modulation protein | gene-EL140_RS05725 |
| AsnLeu84ThrIle | prephenate dehydrogenase | gene-EL140_RS06020 |
| Asn78Ser, Thr72Ala | inositol monophosphatase family protein | gene-EL140_RS06145 |
| His772Asn, Asn770Tyr, Arg768Gln | Ltp family lipoprotein | gene-EL140_RS06460 |
| AlaAspVal352ValAlaIle | sensor histidine kinase | gene-EL140_RS01655 |
| Val24Glu | response regulator | gene-EL140_RS06480 |
| Phe200Tyr | Nif3-like dinuclear metal center hexameric protein | gene-EL140_RS06860 |
| Ter295Lysext a,* | ROK family protein | gene-EL140_RS02070 |
| Ala120Val, ThrIle109LeuLeu | ABC transporter permease | gene-EL140_RS00390 |
| Ser1567Thr, GlyThrGlyAla1549SerThrAsnVal | YSIRK-type signal peptide-containing protein | gene-EL140_RS06920 |
| Ala22Thr | 50S ribosomal protein L23 | gene-EL140_RS08720 |
| GluLeu68GlnAla, Val65Ile, Asn60Glu | phosphoribulokinase | gene-EL140_RS08745 |
| Ala123del | CDP-diacylglycerol—glycerol-3-phosphate 3-phosphatidyltransferase | gene-EL140_RS09460 |
| Pro15Leu | AraC family transcriptional regulator | gene-EL140_RS05010 |
| Met1fs | hypothetical protein | gene-EL140_RS00190 |
a: Numbers refer to amino acid position; * protein sequence extension; fs—frameshift.