Figure 2.

Observations of Chicken embryo fibroblast cell cultures under an inverted microscope exhibiting at 20× (a,b) and 40× (c,d) phase contrast objectives. (a,b). Uninfected and Newcastle disease virus-infected CEF cells at different hours post-infection (hpi). (a) Kadaknath CEF showed delayed cytopathic changes, with cell rounding noticed at 12 h, and (b) Aseel CEF started showing cytopathic changes, such as cell rounding, at 6 h. (c) Normal CEF cells and (d) cytopathic effect of ND virus in CEF showing multinucleated cells (arrow indicates formation of Syncytia).