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. 2023 Jun 27;12(7):1348. doi: 10.3390/antiox12071348

Figure 5.

Figure 5

Ncf190H mice show expansion of macrophages and keratinocytes with NCF1 expression in mannan-induced PsA. (A) Gating strategy for macrophages (CD11b+ F4/80+ Ly6C−) in the spleens by flow cytometry analysis on day ten after intraperitoneal injection of mannan. (B,C) Statistics of macrophage and pDCs (CD45+ CD11C− Ly6C+ B220+ PDCA1+) population in the spleen (n = 5–8). (D,E) Immunohistochemical staining of macrophages (F4/80+) and NCF1 proteins in the ears of mannan-injected Ncf190H mice. Mannan-injected Ncf1m1J mice were used as a positive control, and wild-type Ncf1R90 mice were used as a negative control. Scale bar = 200 μm. (F) Co-staining of NCF1 and macrophages (F4/80+) in ear skin tissue. (G) Co-staining of NCF1 and keratinocytes in skin tissue. Scale bar = 200 μm. (H) Statistics on IHC scores of macrophages (F4/80+) and (I) NCF1 in the skin tissues. (J) Statistics on expression of NCF1 in macrophages (F4/80+) and (K) the ratio of positive keratinocytes in the skin tissues. The data are shown as mean ± SEM (n = 5). * p < 0.05; ** p < 0.01; ns: no significance.