Figure 10.

Peptidomics analysis of antioxidant peptides identified in undigested and in vitro digested Ras cheese at different ripening times. Analysis was carried out on low-molecular weight peptide fractions extracted by ultrafiltration at 3 kDa from undigested and in vitro digested Ras cheese samples at 30 (R30 sample), 90 (R90 sample) and 180 (R180 sample) days of ripening. (a) Number (left y-axis) and total abundance (right y-axis) of antioxidant peptides in undigested Ras cheese sample. (b) Number (left y-axis) and total abundance (right y-axis) of antioxidant peptides in in vitro digested Ras cheese sample. Abundance data are reported as the sum of the intensity of each identified antioxidant peptide measured as area under the peak (AUP) by Skyline analysis. The complete list of identified antioxidant peptides can be found in Supplementary Table S3, whereas the semi-quantitative data can be found in Supplementary Table S4. Different letters among samples in the same assay denote significant differences (p < 0.05).