Figure 11.

Peptidomics analysis of DPP-IV-inhibitory peptides identified in undigested and in vitro digested Ras cheese at different ripening times. Analysis was carried out on low-molecular weight peptide fractions extracted by ultrafiltration at 3 kDa from undigested and in vitro digested Ras cheese samples at 30 (R30 sample), 90 (R90 sample) and 180 (R180 sample) days of ripening. (a) Number (left y-axis) and total abundance (right y-axis) of DPP-IV-inhibitory peptides in undigested Ras cheese sample. (b) Number (left y-axis) and total abundance (right y-axis) of DPP-IV-inhibitory peptides in in vitro digested Ras cheese sample. Abundance data are reported as the sum of the intensity of each identified DPP-IV-inhibitory peptide measured as area under the peak (AUP) by Skyline analysis. The complete list of identified DPP-IV-inhibitory peptides can be found in Supplementary Table S3, whereas the semi-quantitative data can be found in Supplementary Table S4. Different letters among samples in the same assay denote significant differences (p < 0.05).