Table 1.
Reference genes assessed for suitability in qPCR assays of mRNA from equine HF cells.
| Gene Symbol | Forward Primer Sequence (5′-3′) | Reverse Primer Sequence (5′-3′) |
|---|---|---|
| ACTB | CACCTTCTACAACGAGCTGC | CGGGGTGTTGAAGGTCTCA |
| GAPDH | GGAGTCCACTGGTGTCTTCA | GTTCACGCCCATCACAAACA |
| H3F3A | CAAACTTCCCTTCCAGCGTC | TGGATAGCACACAGGTTGGT |
| PPIA | GCATCTTGTCCATGGCGAAT | CAAAGACCACATGCTTGCCA |
| RPL19 | CTGATCATCCGGAAGCCTGT | GGCAGTACCCTTTCGCTTAC |
| SDHA | TGTTGTGTCTCGGTCCATGA | AGATCATGGCCGTCTCTGAA |
| YWHAZ | AGACGGAAGGTGCTGAGAAA | CTTGTGAAGCATTGGGGATCA |
| RNF11 | AGGATAGCTCAAAGAATAGGC | CGGCAGAAATCGAATTGGGT |