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. 2023 Jun 30;12(7):1369. doi: 10.3390/antiox12071369

Figure 3.

Figure 3

Hyperforin induces lipid peroxidation and affects the expression of proteins associated with iron homeostasis. (A) A375, SK-Mel-28, and FO-1 melanoma cells were treated with or without HPF for 48 h, followed by labeling with BODIPY C-11 probe. Fluorescence was measured using cytofluorimetric analysis. (B) Cells were transfected with either HMOX-1 siRNA or scrambled siRNA. After 24 h of transfection, cells were treated with or without 3 µM of HPF. Representative immunoblots showed the expression levels of glutathione peroxidase-4 (GPX-4), solute carrier family 7 member 11 (SLC7A11), ferroptosis suppressor protein 1 (FSP1), transferrin, ferritin heavy chain (FTH), light chain 3 B (LC3B), and GAPDH. Histograms represent the mean values ± S.D. of protein expression levels measured via densitometry from three independent experiments and normalized to GAPDH expression. Student’s t-test was used for unpaired samples to each untreated sample after data normalization. * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001.