FIG. 10.
Rescue of developmental sporulation of mutants unable to aggregate. DZ2, DZ4169 (frzCD), DZ4148 (frzE), and DK1253 (tgl-1) cells were grown and plated for development in 5-μl aliquots at a density of 4 × 109/ml (106/mm2) on CF agar without (top row) or with (bottom row) ampicillin. The plates were incubated at 28°C, and the images were taken after 1 week. Ampicillin was added to a final concentration of 100 μg/ml. The spores were harvested and counted as described in Materials and Methods. Bar = 1 mm. Numbers of spores are shown below each image.