FIG. 5.

Sporulation of cells pregrown in ampicillin. DZ2 was prepared for development in MMC in submerged culture in 35-mm-diameter petri dishes as described in Materials and Methods. The number of spores reported reflects the total number of spores for each sample. (A) Sporulation at 34°C. Open squares, cells grown in CYE lacking ampicillin; solid triangles, cells grown in CYE containing 100 μg of ampicillin/ml. (B) Sporulation at 28°C. Open circles, cells grown in CYE lacking ampicillin; solid circles, cells grown in CYE containing 100 μg of ampicillin/ml.