FIG. 7.

Transmission electron microscopy of DZ2 spores. Spores were harvested and prepared for transmission electron microscopy as described in Materials and Methods. (A) Spore formed in suspension in CYE by the addition of 0.5 M glycerol; incubation was for 24 h. (B) Spore formed in suspension in MMC by the addition of 1 mM ampicillin (372 μg/ml); incubation was for 24 h. (C) Enlargement of wall region of the cell in panel A. (D) Enlargement of wall region of the cell in panel B. (E) Fruiting body spore formed in submerged culture in MMC lacking ampicillin. (F) Fruiting body spore formed on MMC containing 100 μg of ampicillin/ml. (G) Enlargement of wall of the cell in panel E. (H) Enlargement of wall of the cell in panel F. Bars = 1 μm.