TABLE 2.
Sporulation of DK1622a
| Elapsed time (days) | CF
|
CF + d-cycloserine
|
||
|---|---|---|---|---|
| Stage of development | No. of spores | Stage of development | No. of spores | |
| 1 | No mounds | <102 | Tight mounds | 1 × 103 |
| 2 | Tight mounds | 2 × 106 | Fruiting bodies | 3 × 106 |
| 3 | Fruiting bodies | 3 × 106 | Fruiting bodies | 3 × 106 |
a
DK1622 cells were prepared for development in submerged culture in CF medium lacking pyruvate in 12-well tissue culture dishes as described in Materials and Methods, with 1 ml of medium/well. The plates were incubated at 28°C. The cells were scraped from the plates, pelleted by centrifugation, resuspended in 0.1 ml of water, sonicated to disrupt fruiting bodies for 15 cycles at 90% duty cycle at a setting of 1 with a microtip on a Branson model 450 Sonifier (approximately 10 W of power). Spores were counted in a hemacytometer after dilution in water, if necessary.