TABLE 5.
Medium | Spores/plate |
---|---|
MMC (no Casitone) | 4 × 107 |
MMC, 0.2% Casitone | 4 × 104 |
MMC, 0.2% Casitone + d-cycloserine (5 μg/ml) | 2 × 105 |
MMC, 0.2% Casitone + d-cycloserine (10 μg/ml) | 4 × 105 |
MMC, 0.2% Casitone + phosphomycin (5 μg/ml) | 1 × 105 |
MMC, 0.2% Casitone + phosphomycin (15 μg/ml) | 2 × 105 |
DK1622 cells were grown and plated for development in submerged culture in 100-mm-diameter petri plates as described in Materials and Methods. The plates were incubated at 28°C for 1 week before the cultures were scraped from the plate and sonicated to release spores from fruiting bodies. The spores were counted in a hemacytometer.