Figure 3.

Protein glutathionylation is evident in (a) primary intestinal tissue samples resected for surgical NEC (* p-value 0.0417, Control N = 3, NEC N = 3), as well as (b) human enteroid cultures after 36 h of in vitro NEC induction (* p-value 0.0119, N = 4 separately grown and treated, pooled enteroid culture samples per group. Each enteroid culture sample is a pooled sample of 3 enteroid domes that includes approximately 150 enteroids grown to maturity in a three-dimensional matrix dome). Analysis carried out with Student’s t-test and ANOVA, respectively, with p-value < 0.05 considered significant.