. 2023 Jul 11;13(14):2265. doi: 10.3390/ani13142265

Table 2.

Primer sequences of the different genes analyzed and their RT-PCR conditions.

Gene	Access. Number	Primer	Nucleotide Sequence 5′-3′	Annealing T (°C)	Reference
nfΚβ2	KM225790	Fw	CTGGAGGAAACTGGCGGAGAAGC	60	[49]
nfΚβ2	KM225790	Rv	CAGGTACAGGTGAGTCAGCGTCATC	60	[49]
il-1b	AJ53742	Fw	ATTACCCACCACCCACTGAC	60	[50]
il-1b	AJ53742	Rv	TCTCTTCCACTATGCTCTCCAG	60	[50]
hif-1a	DQ171936	Fw	GACTTCAGCTGCCCTGATTC	60	[51]
hif-1a	DQ171936	Rv	GGCTGGTTTATAGCGCTGAG	60	[51]
nd5	KF857307	Fw	CCCGATTTCTGTGCCCTACTA	60	[52]
nd5	KF857307	Rv	AGGAAAGGAGTGCCTGTGA	60	[52]
cyb	EF427553	Fw	TGCCTACGCTTCCTTCGCTCGATCC	60	[53]
cyb	EF427553	Rv	TAACGCCAACACCCCGCCCAAT	60	[53]
cox	KF857308	Fw	ATACTTCACATCCGCAACCATAA	60	[53]
cox	KF857308	Rv	AAGCCTCCGACTGTAAATAAGAAA	60	[53]
ucp1	MH138003	Fw	CGATTCCAAGCCCAGACGAACCT	60	[53]
ucp1	MH138003	Rv	TGCCAGTGTAGCGACGAGCC	60	[53]
sod	FJ860004.1	Fw	CATGTTGGAGACCTGGGAGA	60	[54]
sod	FJ860004.1	Rv	TGAGCATCTTGTCCGTGATGT	60	[54]
cat	FJ860003.1	Fw	TGGGACTTCTGGAGCCTGAG	60	[54]
cat	FJ860003.1	Rv	GCAAACCTCGATCGCTGAAC	60	[54]
gpx	FM013606.1	Fw	AGTTCGTGCAGTTAATCCGGA	60	[54]
gpx	FM013606.1	Rv	GCTTAGCTGTCAGGTCGTAAAAC	60	[54]
tnf-1α	DQ200910.1	Fw	GCCAAGCAAACAGCAGGAC	60	[52]
tnf-1α	DQ200910.1	Rv	ACAGCGGATATGGACGGTG	60	[52]
casp-9	DQ345775	Fw	GGCAGGACTCGACGAGATAG	62.7	[55]
casp-9	DQ345775	Rv	CTCGCTCTGAGGAGCAAACT	62.7	[55]
α-tub (hk)	AY326429.1	Fw	AGGCTCATTGGCCAGATTGT	60	[31]
α-tub (hk)	AY326429.1	Rv	CAACATTCAGGGCTCCATCA	60	[31]
rpl17	AF139590	Fw	GAGGACGTGGTGGTTCATCT	60	[56]
rpl17	AF139590	Rv	CTGGCTTGCCTTTCTTGACT	60	[56]

Fw: Forward primer sequence, Rv: Reverse primer sequence.