Figure 2.

CREB upregulates the promoter activities of ACSL1 through two binding sites. (A) Overexpression efficiencies of vectors containing the coding sequences of the two isoforms of porcine CREB; (B) Overexpression of both isoforms of CREB enhances promoter activity. (C) The putative binding sites of CREB positively regulate promoter activity. Scheme for deleting the binding sites of CREB is provided below, ^ indicates the nucleotide was deleted; (D) The promoting effects of CREB on promoter activity were reversed by inactivation of the binding sites. The values were compared with that in cells cotransfected with a wild type reporter gene and plasmids overexpressing CREB; (E) Ectopic CREB increases the mRNA level of ACSL1. (F) Ectopic CREB increases the protein level of ACSL1. * p < 0.05, ** p < 0.01.