Figure 6.

Effect of DGK inhibitors on the HL-60 cell cycle. HL-60 cells were treated with DMSO (1%), R59022 (25 µM), or R59949 (100 µM) and incubated for 24 h before DraQ5 staining. Black line: experimentally observed data; purple line: data estimated by the analysis model; blue: estimated G1 phase; yellow: estimated S phase; green: estimated G2 phase. Representative experiment indicating cells treated with (A) DMSO, (B) R59022, or (C) R5949. (D) Percentage of cells in each phase of the cell cycle for each treatment. Data are expressed as the mean ± SD of five independent experiments. * t-test, p < 0.05.