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. 2023 Jul 3;11(7):1885. doi: 10.3390/biomedicines11071885

Figure 2.

Figure 2

Effect of Carbamathione on the expression of Hsp 27, P-AKT, and AKT after glutamate (10 mM) treatment. Western blot analysis using antibodies against Hsp 27 (a) and P-AKT, AKT (b). The bar graphs reflect the densitometry data from the respective experiments for Hsp 27 (n = 6) (a), P-AKT, AKT (b), and P-AKT/AKT (c) (F (3, 14) = 9.226; p = 0.0013 (n = 5)) Western blot. Note: 1—control, 2—glutamate (10 mM for 24 h), 3—Carbamathione (25 µM for 1 h) followed by glutamate (10 mM for 24 h), and 4—Carbamathione (25 µM for 24 h) with glutamate (10 mM for 24 h). (All data are presented as mean ± SEM, where #/* p < 0.05, ##/** p < 0.01, ###/*** p < 0.001. Pound signs (#) indicate statistical significance between groups 1 and 2; asterisks (*) indicate statistical significance between groups 2 and 3 and between groups 2 and 4).