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. 2023 Jul 3;11(7):1885. doi: 10.3390/biomedicines11071885

Figure 5.

Figure 5

Effect of Carbamathione on the expression of Hsp 70, Hsp 27, P-AKT, AKT, and P-STAT 3 after hypoxia/reoxygenation treatment. Western blot analysis using antibodies against Hsp 70 (a), Hsp 27 (b), P-AKT, AKT (c), and P-STAT 3 (d). The bar graphs reflect the densitometry data from the respective experiments for Hsp 70, Hsp 27 (a,b), P-AKT, AKT (c), P-AKT/AKT ((e) (F (2, 7) = 5.097; p = 0.0430 (n = 5)), and P-STAT 3 (d) Western blots. Hypoxia (Column 2) = hypoxia (0.3% O2) for 24 h, reoxygenation for 24 h; Carb + hypoxia: cells were treated with 25 µM Carbamathione for 1 h, then hypoxia for 24 h, and reoxygenation for 24 h. Note: 1—control (Normoxia 48 h); 2—hypoxia/reoxygenation (48 h); and 3—hypoxia/reoxygenation (48 h) after 1 h, 25 µM Carbamathione preincubation. (All data are presented as mean ± SEM (n = 5); #/* p < 0.05, ## p < 0.01, and *** p < 0.001. Pound signs (#) indicate statistical significance between groups 1 and 2; asterisks (*) indicate statistical significance between groups 2 and 3).