TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| C. jejuni | ||
| NCTC 11168 | Parental strain | National Collection of Type Cultures |
| AV63 | 11168 perR::Kanr | This study |
| AV42 | 11168 fur::Cmr | 31 |
| AV67 | 11168 perR::Kanrfur::Cmr | This study |
| E. coli DH5α | F− φ80dlacZΔM15 | Gibco BRL |
| Plasmids | ||
| pBluescript II SK− | Cloning vector, Apr | Stratagene |
| pJMK30 | C. coli kanamycin (Kanr) resistance cassette in pUC19 | 31 |
| pAV205 | 1.4-kb PCR-amplified fragment of C. jejuni NCTC 11168 containing the perR region in pBluescript | This study |
| pAV214 | SmaI-cut Kanr cassette of pJMK30 cloned in the EcoRV site in perR in pAV205; the Kanr cassette is in the reverse orientation with respect to perR | This study |