Figure 1.

The overall workflow of the crisprQTL toolkit: (1) Transduction: lentiviral delivery of sgRNAs library designed to target candidate regions and the generation of stable cell lines at high multiplicity of infection (MOI). (2) Droplet generation: after screening and selection for the phenotype of interest, polyclonal cells go through droplet generation. (3) Lysing and barcoding: cell lysis is followed by reverse transcription and barcoding. (4) Library generation: pooling samples is followed by PCR to prepare the library ahead of sequencing. (5) Data analysis: data processing, mapping, and assigning sgRNAs to cells.