TABLE 3.
Complementation of E. coli mutants of ferric enterochelin esterase (AN272 fes) and ferric enterochelin permease (AB1515.718 fepD and AB1515.764 fepG)a
Plasmid used for complementation | Complementation of mutant at concn (μM) of 2,2′-dipyridyl
|
||
---|---|---|---|
AN272 fes, 200 | AB1515.718 fepD and AB1515.764 fepG
|
||
100 | 200 | ||
pACYC184 | φ | φ | φ |
pBluescript KS(+) | φ | φ | φ |
pSI10 | + | + | φ |
pH-1 | + | + | φ |
pH-1fes::Tn552kan | φ | ND | ND |
pH-1fepD::Tn552kan | ND | φ | φ |
Lack of complementation (φ) or positive complementation (+) by the bioassay as described in Materials and Methods. ND, not determined.