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. 2023 Jul 6;13(7):712. doi: 10.3390/bios13070712

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Workflow of spatial transcriptomics sequencing methods: Schematic of the workflow of the (a) ST method. Reprinted from ref. [17]. (b) Slide-seq: (i) schematic of array generation and (ii) schematic of sample preparation. Reprinted from ref. [18]. (c) HDST method: Barcoded bead array generation process. Reprinted from ref. [19]. (d) Seq-Scope: Different HDMI cluster formation processes and HDMI arrays capture RNA released from the overlying frozen section. Reprinted from ref. [20]. (e) sci-Space: Arrayed single-stranded oligos transferring to nuclei and imaging process. Reprinted from ref. [21]. (f) Stereo-seq: The whole pipeline of Stereo-seq, including DNB-patterned array chip design, spatial coordinates sequencing, capture probe preparation, in situ RNA capture, library construction and sequencing, and finally the data analysis process. Reprinted from ref. [22]. (g) Pixel-seq: Schematic of amplifiable DNA stamping. Reprinted from ref. [23].

Workflow of spatial transcriptomics sequencing methods: Schematic of the workflow of the (a) ST method. Reprinted from ref. [17]. (b) Slide-seq: (i) schematic of array generation and (ii) schematic of sample preparation. Reprinted from ref. [18]. (c) HDST method: Barcoded bead array generation process. Reprinted from ref. [19]. (d) Seq-Scope: Different HDMI cluster formation processes and HDMI arrays capture RNA released from the overlying frozen section. Reprinted from ref. [20]. (e) sci-Space: Arrayed single-stranded oligos transferring to nuclei and imaging process. Reprinted from ref. [21]. (f) Stereo-seq: The whole pipeline of Stereo-seq, including DNB-patterned array chip design, spatial coordinates sequencing, capture probe preparation, in situ RNA capture, library construction and sequencing, and finally the data analysis process. Reprinted from ref. [22]. (g) Pixel-seq: Schematic of amplifiable DNA stamping. Reprinted from ref. [23].