Figure 2.
SPR results (A): Example SPR traces from c(WWWRRR) interaction (from 4 to 128 µM) with DMPC:PG liposomes. Red points indicate values used for steady-state fitting (to obtain KD and KP). The grey rectangle indicates the dissociation process which is used to obtain koff, according to Figuera et al. (2017) [32]. (B): Partitioning constants KP. Full, empty, and grey bars correspond to DMPC, DMPC:PG (95:5), and DMPC:LPS (90:10) liposomes, respectively. (C): Dissociation rate koff. (D): Association rate kon, which is obtained by calculation using measured KD (Figure S1) and kon. Values used in the plot are available in Table S1. Values for DMPC and DMPC:PG interaction are reproduced from [45].
